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This blog was designed for the Biomedical Technology students at the Durban University of Technology, in Durban, South Africa. It consists of short notes on aspects that I feel that my students grapple with, and aims to provide a better explanation than that they would receive in lectures. It is also a very personal blog, where I feel comfortable 'talking' to my students.

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Wednesday, March 17, 2010

identification of Staphylococcus and Streptococcus



these orgs are Gram positive cocci. Strept occur either in chains or as diplococci. Staph occur in grape like clusters.

to differentiate between Staph and Strept
catalase test (3% H2O2)
Staph is pos (immediate bubbling when org is added)
Strept is neg (no immediate bubbling when org is added)

to differentiate between S aureus and S epidermidis
DNAse test (stab inoculation)
S aureus is pos (clear zone around colonies after 1N HCl is added)
S epidermidis is neg (medium turns opaque around colonies after 1N HCl is added)

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