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This blog was designed for the Biomedical Technology students at the Durban University of Technology, in Durban, South Africa. It consists of short notes on aspects that I feel that my students grapple with, and aims to provide a better explanation than that they would receive in lectures. It is also a very personal blog, where I feel comfortable 'talking' to my students.

Please email me sherlien@dut.ac.za




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Monday, February 14, 2011

stool processing (prac reports)

i am disappointed at the lack of quality and thoroughness of your research for the practical report.
Many students chose the easiest pathogens. If you dont consider the unusual or different pathogens, how are you going to learn?
Insufficient research was done on the identification of each pathogen chosen. Please consult relatively new textbooks for information
In your Gram of the culture and the specimen, you need to be specific about the morphology of the pathogens. It isnt enough to say the organism is a GNB. Many organisms have very specific shapes, an the fact that you have not indicated so, proves that you havent done sufficient reading and research. It would also be a good idea to do a simple pencil drawing to show the shapes of these organisms.
The layout of the practical reports needs work. It seems as if the information is just gathered and written down with no forethought. Subheadings are important. Just as important is the specific tasks/processing done on each day. If you dont have a clear idea of what is done on which day, please either ask me or do some extra reading.
When you have chosen your pathogens, it is important to specify a species. Shigella has 4 species, and these need to be distinguished from each other. Not all species cause severe disease.
If you have written about agar/culture plates we did not learn about in micro 1, you need to indicate the use/purpose of the plate, its main ingredients, and the expected morphology of the pathogen on that plate.
There are some students who are confused about Gram reaction and culture morphology on the agar plates.

These are some of the most important points Ive picked up from your prac reports. Please ensure that these errors are not repeated in the next prac report.

possible pathogens in stools

possible pathogens:

Vibrio cholerae
Yersinia enterocolitica
Shigella dysenteriae
Salmonella typhi/paratyphi/enteriditis
Bacillus cereus
Campylobacter jejuni
Aeromonas