Welcome Dear Student

This blog was designed for the Biomedical Technology students at the Durban University of Technology, in Durban, South Africa. It consists of short notes on aspects that I feel that my students grapple with, and aims to provide a better explanation than that they would receive in lectures. It is also a very personal blog, where I feel comfortable 'talking' to my students.

Please email me sherlien@dut.ac.za




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Wednesday, April 7, 2010

To think about....

Why would you recommend to a friend that he places 5, 10 and 20 cent
coins in his pet’s water bowl?
Why can someone get very ill from eating a lot of cold meats all at once?
Why is silver nitrate put into the eyes of newborns?

TRANSMISSION OF DISEASE

Categorised as either vehicle, vector and contact
Vehicle is non living
Vector is living
Contact is you know???
Mechanical vehicle is the insect itself ex allergy to bee sting
Biological vehicle is when part of life cycle of pathogen is inside insect ex malaria
Example of Waterborne vector is cholera
For pathogens to be transmitted though air, the distance must be less than 1 meter
Foodborne pathogen transmitted through cooking utensils, food itself, etc
Direct contact is kissing, sex
Indirect contact is through fomites
Droplets are when nasty people sneeze and cough directly on you

Media tut questions

Choose the odd one out, and justify your choice
1. Peptone, asparagines, L cysteine, glucose
2. Malachite green, glycerol, asparagines, methylene blue
3. Ferric citrate, magnesium citrate, ferric ammonium citrate
4. Increased dextrose, Proteus, decrease Ph, Rhizopus
5. Rabbit blood, trimethoprim, penicillin, Bordet Gengou
6. Sterile mineral oil, closed, Pseudomonas, fermentative
7. Trimethoprim, vancomycin, nystatin, colistin
8. Ox bile, brilliant green, K tellurite, blood
9. Bile salts, ox bile, sodium citrate
10. Black colour in oxidase, acid slant in TSI, red layer at top of SIM, negative citrate
11. E coli, Klebsiella, Proteus, Salmonella
12. Bacillus, Staphylococcus, Neisseria, E coli, Klebsiella
13. Pseudomonas, E coli, Alcaligenes, Streptococcus
14. 40%KOH, alpha naphthol, MRVP, p dimethylaminobenzaldehyde, methyl red reagent
15. Pink urease, low electrolytes, PAD pos, alkaline slant

MEDIA Tuts

Explain the relationship between:
Methyl red reagent; MRVP; acidic end products; pH6; Enterobacter aerogenes
Voges Proskauer; MRVP; non acidic end products; pH4; Escherichia coli

YOU NEED TO KNOW:
MRVP is the medium. It comprises 2 tests, MR and VP.
Both tests are used to differentiate between E coli and Enterobacter aerogenes.
Both orgs degrade glucose and form by products (E coli at pH4, and Ent at pH6)
Mr tests for pH4 and VP tests for pH6. In both tests a red colour is positive.
E coli end products are called acidic
Ent end products are called non acidic.
Therefore E coli is MR pos and VP neg
Enterobacter is MR neg and VP pos