all stools inoculated onto XLD, Mac, Selenite F broth, Campy BA or Butzlers for Campy
patient less than 2 years: stain for Cryptosporidium, media for EPEC
Loose stools: TCBS for Vibrio, Sorbital Mac for E coli 0157H7
bloody stools: sorbitol Mac
0157H7 is sorbitol negative (pale colonies) whilst other E coli are sorbitol pos (pink colonies)
query Campylobacter: Campy BA or Butzlers or Skirrows
Campy BA contains sheep blood, trimethoprim, vancomycin, cephalothin, amphotericin B and Polymyxin B
Skirrows contains 7% lysed horse blood, vancomycin, polymyxin B, trimethoprim
all plates for Campy incubated microaerophically at 42 degrees for up to 5 days.
No comments:
Post a Comment