after removing from the incubator, add solution A and B (in that order, 5 drops each) to each tube. Wait for a few minutes for the colour to develop. A red colour indicates a positive reaction, i.e. reduction of nitrate to nitrite.
zinc is added to all negative tubes. Zinc is able to reduce nitrates to nitrites, therefore a development of a red colour here indicates a negative reaction.
the tube that still shows no colour change is..... wait for it........ positive for nitrate reduction.
why you ask??????????
simply silly
because the organism reduced nitrates to ammonia gas. You could try smelling it to be sure, but I wouldn't advise it. Apart from fainting and becoming a hazard on the floor, (it's nasty down there), I can't see myself picking you up. Duh!!!!
isn't all this stuff amaaaazing!!!!
sure i... zzz *snoring*
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