gelatin has properties of solids at 4 deg and liquids at 37 deg. After inoculation and incubation, all tubes are liquid. We are trying to determine whether the org produces gelatinase, which will degrade the gelatin. After removing from the incubator, place in a fridge for 20 min. REmove and then read as follows:
solid = negative for gelatinase activity
liquid = positive for gelatinase activity
NB! Even a little liquid or semi solid appearance is positive
so what happened and why??
orgs that produce gelatinase break down the gelatin, so it will not solidify at 4 deg. Gelatin will liquify at 37 deg, and solidify at 4 deg, so is solid when removed from fridge.
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