urine processing DAY ONE

day one
macroscopy of specimen
describe odour (if applicable) colour, turbidity, volume
microscopy of specimen
decant few ml into a centrifuge tube
centrifuge and make wet prep with sediment
examine using up to 40X obkective
examine for cells, crystals, casts, and bacteria/yeasts
if casts and crystal seen, name type (significant as it denotes type of kidney damage)
culture of specimen
use plates dependent on what seen in wet prep (use urine from original urine specimen container)
include BA (enriched medium, usually all orgs grow), Mac for LF/NLF (GNB), CLED (total colony count, streak for single colonies without flaming, use 1/1000 ul loop)
include SAB if yeasts seen in wet prep
ALWAYS remember potential pathogens found in urine
perform an antimicrobial test to dtermine if px is on antimicrobial therapy
make a suspension of B subtilis equivalent to McFarlands 0.5
seed a CAB plate using the suspension
place a sterile filter paper disc on plate
place a loopful (1/100 ul) of urine on disc
incubate all plates at 37 degrees, aerobically for 18-24 hours
lastly do a dipstick to determine any abnormal chemistry.
NB! Nitrites indicate bacterial activity i.e. reduction of nitrates