urine processing DAY TWO Biochemical tests

Staphylococcus id
S saprophyticus is common cause of UTI in women and in men with indwelling catheters
only difference between S saprophyticus is its resistance to 5ug novobiocin
S saprophyticus is coag neg, MSA (pink colonies), DNA neg and novobioicin resistant.
so set up
DNA plate (stab and streak)
MSA (streak for single colonies)
coagulase (inoculate plasma with colony, incubate for up to 4 hours, remove and refridgerate if unable to read on same day)
seed CAB plate with suspension of org equivalent to McFarlands 0.5. Place novobiocin disc on plate)

GNB id
inoculate
TSI (stab and streak\
SIM
citrate *stab and streak)
PAD
urease

yeast id
set up germ tube
inoculate serum with colony
incubate at 37 degrees for up to 2 hours
make wet prep and examine for germ tubes which is pos for C albicans

Enterococcus id
inoculate BA plate. Place bacitracin disc in area of most growth (should be resistant)
inoculate aesculin bile (pos for Enterococcus)