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This blog was designed for the Biomedical Technology students at the Durban University of Technology, in Durban, South Africa. It consists of short notes on aspects that I feel that my students grapple with, and aims to provide a better explanation than that they would receive in lectures. It is also a very personal blog, where I feel comfortable 'talking' to my students.

Please email me sherlien@dut.ac.za




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Wednesday, February 9, 2011

urine processing DAY TWO

examine antimicrobial plate
any zone around disc indicates that the px is on antibiotics and will influence the significance of any growth of organisms
examine CLED plate
estimate the amount of growth
growth up to the squiggle in the middle of plate equals 100 000 orgs per ml of urine
growth up to a level below the squiggle decreases by a power of 10. (please see me if you dont understand this)

examine the BA Growth should represent totality of all orgs seen on other plates
examine MaC for LF/NLF (LF are pink, NLF are brown, transparent, colourless)
examine SAB for yeasts (distinctive odour)

NB! you must get used to the smell of bacteria. Orgs like yeasts, E coli, Klebs, S aureus have very distinct smells. Smells is one way of guiding you to a possible identity of the organism.

set up biochemical tests to identify any potential pathogens seen.
contd in next post

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