take 5 testtubes and label as A, B, O, a and b.
A, B and O are the forward grouping tubes. Its purpose is to determine the identity of antigens present. Antigens are found on the surface of RBC, so the patients red cells are added to these tubes. The known is the antibodies, each of which is colour coded.
a and b is the reverse typing. Its purpose is to determine the identity of any antibodies present. Ab are found in serum/plasma, so the patients plasma are added to these tubes. The known are the antigens, which are red in colour.
dispense 1 drop of a 3% RBC suspension to tubes A, B,O
Dispense 1 drop of plasma to tubes a, b
add 1 drop of the relevant Ab and Ag to each tube (e.g.antiA to Tube A, etc)
mix well and incubate at room temperature for 15 minutes
examine each tube for agglutination
record your results and determine the ABO blood type
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