dispense approx 0.5ml of RBC to a testtube
fill the tube up to 3/4 with saline
parafilm and mix by inverting tube once
centrifuge at 2000rpm or 0.5g for 5 minutes
remove parafilm, remove saline
repeat above steps twice more (total of 3 times)
at end of last centrifugation, remove as much saline without disturbing the RBC
add saline up to 1/2 tube, mix and note the colour
we are looking for a bright red/scarlet colour
if the suspension is too dark, add more saline and examine again
once the scarlet colour is achieved, use the suspension in your test
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